SrasV1, Main, Exploration, bibRecord, 003098

Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice▿

Identifieur interne : 003098 ( Main/Exploration ); précédent : 003097; suivant : 003099

Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice▿

Auteurs : Baojing Lu ; Ling Tao ; Ting Wang ; Zhenhua Zheng ; Bao Li ; Ze Chen ; Yi Huang ; Qinxue Hu ; Hanzhong Wang

Source :

Clinical and Vaccine Immunology : CVI [ 1556-6811 ] ; 2008.

RBID : PMC:2620671

Descripteurs français

KwdFr :
- Animaux, Anticorps antiviraux (sang), Cytométrie en flux, Femelle, Interféron gamma (biosynthèse), Interleukine-2 (biosynthèse), Lymphocytes T (immunologie), Protéines virales (génétique), Protéines virales (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (immunologie), Techniques immunoenzymatiques (), Vaccins à ADN (immunologie), Virus du SRAS (génétique), Virus du SRAS (immunologie).
MESH :
- biosynthèse : Interféron gamma, Interleukine-2.
- génétique : Protéines virales, Virus du SRAS.
- immunologie : Lymphocytes T, Protéines virales, Syndrome respiratoire aigu sévère, Vaccins à ADN, Virus du SRAS.
- sang : Anticorps antiviraux.
- Animaux, Cytométrie en flux, Femelle, Souris, Souris de lignée BALB C, Techniques immunoenzymatiques.

English descriptors

KwdEn :
- Animals, Antibodies, Viral (blood), Female, Flow Cytometry, Immunoenzyme Techniques (methods), Interferon-gamma (biosynthesis), Interleukin-2 (biosynthesis), Mice, Mice, Inbred BALB C, SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), T-Lymphocytes (immunology), Vaccines, DNA (immunology), Viral Proteins (genetics), Viral Proteins (immunology).
MESH :
- chemical , biosynthesis : Interferon-gamma, Interleukin-2.
- chemical , blood : Antibodies, Viral.
- genetics : SARS Virus, Viral Proteins.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Vaccines, DNA, Viral Proteins.
- methods : Immunoenzyme Techniques.
- Animals, Female, Flow Cytometry, Mice, Mice, Inbred BALB C.

Abstract

Vaccine development for severe acute respiratory syndrome coronavirus (SARS-CoV) has mainly focused on the spike (S) protein. However, the variation of the S gene between viruses may affect the efficacy of a vaccine, particularly for cross-protection against SARS-like CoV (SL-CoV). Recently, a more conserved group-specific open reading frame (ORF), the 3a gene, was found in both SARS-CoV and SL-CoV. Here, we studied the immunogenicity of human SARS-CoV 3a and bat SL-CoV 3a DNA vaccines in mice through electroporation immunization followed by enzyme-linked immunosorbent, enzyme-linked immunospot, and flow cytometry assays. Our results showed that high levels of specific humoral responses were induced by SARS-CoV 3a and SL-CoV 3a DNA vaccines. Furthermore, a strong Th1-based cellular immune response was stimulated by both DNA vaccines. The vaccines stimulated gamma interferon production mainly by CD8⁺ T cells and interleukin-2 (IL-2) mainly by CD4⁺ T cells. Of interest, the frequency of IL-2-positive cells elicited by the SARS-CoV 3a DNA vaccine was significantly higher than that elicited by the SL-CoV 3a DNA vaccine. In summary, our study provides a reference for designing cross-protective DNA vaccines based on the group-specific ORFs of CoVs.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620671

DOI: 10.1128/CVI.00261-08
PubMed: 18987164
PubMed Central: 2620671

Affiliations:

Le document en format XML

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<term>Flow Cytometry</term>
<term>Immunoenzyme Techniques (methods)</term>
<term>Interferon-gamma (biosynthesis)</term>
<term>Interleukin-2 (biosynthesis)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
<term>T-Lymphocytes (immunology)</term>
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<term>Viral Proteins (genetics)</term>
<term>Viral Proteins (immunology)</term>
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<term>Anticorps antiviraux (sang)</term>
<term>Cytométrie en flux</term>
<term>Femelle</term>
<term>Interféron gamma (biosynthèse)</term>
<term>Interleukine-2 (biosynthèse)</term>
<term>Lymphocytes T (immunologie)</term>
<term>Protéines virales (génétique)</term>
<term>Protéines virales (immunologie)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Techniques immunoenzymatiques ()</term>
<term>Vaccins à ADN (immunologie)</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (immunologie)</term>
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<term>Interleukin-2</term>
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<term>Interleukine-2</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
<term>Viral Proteins</term>
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<term>Virus du SRAS</term>
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<term>Protéines virales</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Vaccins à ADN</term>
<term>Virus du SRAS</term>
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<term>Severe Acute Respiratory Syndrome</term>
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<term>Vaccines, DNA</term>
<term>Viral Proteins</term>
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<front><div type="abstract" xml:lang="en"><p>Vaccine development for severe acute respiratory syndrome coronavirus (SARS-CoV) has mainly focused on the spike (S) protein. However, the variation of the S gene between viruses may affect the efficacy of a vaccine, particularly for cross-protection against SARS-like CoV (SL-CoV). Recently, a more conserved group-specific open reading frame (ORF), the 3a gene, was found in both SARS-CoV and SL-CoV. Here, we studied the immunogenicity of human SARS-CoV 3a and bat SL-CoV 3a DNA vaccines in mice through electroporation immunization followed by enzyme-linked immunosorbent, enzyme-linked immunospot, and flow cytometry assays. Our results showed that high levels of specific humoral responses were induced by SARS-CoV 3a and SL-CoV 3a DNA vaccines. Furthermore, a strong Th1-based cellular immune response was stimulated by both DNA vaccines. The vaccines stimulated gamma interferon production mainly by CD8<sup>+</sup>
 T cells and interleukin-2 (IL-2) mainly by CD4<sup>+</sup>
 T cells. Of interest, the frequency of IL-2-positive cells elicited by the SARS-CoV 3a DNA vaccine was significantly higher than that elicited by the SL-CoV 3a DNA vaccine. In summary, our study provides a reference for designing cross-protective DNA vaccines based on the group-specific ORFs of CoVs.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name>
<name sortKey="Hu, Qinxue" sort="Hu, Qinxue" uniqKey="Hu Q" first="Qinxue" last="Hu">Qinxue Hu</name>
<name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name>
<name sortKey="Li, Bao" sort="Li, Bao" uniqKey="Li B" first="Bao" last="Li">Bao Li</name>
<name sortKey="Lu, Baojing" sort="Lu, Baojing" uniqKey="Lu B" first="Baojing" last="Lu">Baojing Lu</name>
<name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name>
<name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name>
<name sortKey="Wang, Ting" sort="Wang, Ting" uniqKey="Wang T" first="Ting" last="Wang">Ting Wang</name>
<name sortKey="Zheng, Zhenhua" sort="Zheng, Zhenhua" uniqKey="Zheng Z" first="Zhenhua" last="Zheng">Zhenhua Zheng</name>
</noCountry>
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Serveur d'exploration SRAS

Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice▿

Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice▿

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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